Journal: JCI Insight

Article Title: Matrix metalloproteinases are hallmark early biomarkers and therapeutic targets in FSHD

doi: 10.1172/jci.insight.195104

Figure Lengend Snippet: ( A ) UMAP plot of merged single-cell data from uninduced WT (control) and dox-induced iDUX4 mice (10 days on 625 mg/kg dox chow), with clusters annotated by cell types. ( B ) UMAP comparison of cell clusters between control and DUX4-induced muscles. ( C ) Proportional representation of major cell populations in control and DUX4-induced muscles. ( D and E ) UMAP plots showing the spatial distribution and cellular origin of MMP expression in control ( D ) and DUX4-induced ( E ) muscles. ( F ) Bubble plots highlighting distinct MMP expression patterns across cell types. ( G ) Normalized fold-change in MMP expression within selected cell populations. ( H ) Violin plots showing expression levels of Mmp2 , Mmp14 , and Mmp19 across annotated cell clusters. Data are presented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 by Student’s 2-tailed t test. ECs, endothelial cells; MSCs, mesenchymal stem cells; SCs, satellite cells; UMAP, uniform manifold approximation and projection.

Article Snippet: For immunofluorescence, tissue sections or sorted FAPs were fixed in 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 for 30 minutes, and incubated overnight at 4°C with primary antibody against collagen VI (1:200, Proteintech), MMP2 (1:500, Proteintech), MMP14 (1:500, Proteintech), PDGFRα (1:200, BioLegend), F4/80 (1:200, BioLegend), and Ki-67 (1:400, BioLegend), followed by secondary antibody conjugate to Alexa Fluor 488, 555, or 647 (1:500, Thermo Fisher Scientific) for 2 hours at room temperature.

Techniques: Control, Comparison, Muscles, Expressing

Journal: The American Journal of Pathology

Article Title: Laminin-γ2–NR6A1 Fusion Protein Promotes Metastatic Potential in Non–Small-Cell Lung Carcinoma Cells without Epidermal Growth Factor Receptor Mutation

doi: 10.1016/j.ajpath.2025.03.006

Figure Lengend Snippet: Lm-γ2F expression enhances matrix metalloproteinase (MMP) production and epidermal growth factor receptor (EGFR) downstream signaling in lung cancer cells. A: Effect of enforced Lm-γ2F expression on MMP secretion in EKVX cells, analyzed using gelatin zymography. B: Effect of enforced Lm-γ2F expression on membrane-type 1 MMP (MT1-MMP) levels in EKVX cell lysates, detected by Western blot analysis using anti–MT1-MMP polyclonal antibodies. C: Effect of gefitinib on MT1-MMP expression induced by Lm-γ2F in EKVX cells. D: Effect of enforced Lm-γ2F expression on EGFR and downstream phosphorylation of extracellular signal-regulated kinase (ERK) and AKT in EKVX cell lysates. EKVX cells were cultured in 0.1% fetal calf serum for 24 hours, and cell lysates were subjected to Western blot analysis. CBB, Coomassie Brilliant Blue R-250; DMSO, dimethyl sulfoxide.

Article Snippet: MT1-MMP , Rabbit , Millipore , AB6004 , 1:2000.

Techniques: Expressing, Zymography, Membrane, Western Blot, Phospho-proteomics, Cell Culture